First detection of the presence of naturally occurring grapevine downy mildew in the field by a fluorescence-based method.

Abstract

Early detection of fungal pathogen presence in the field would help to better time or avoid some of the fungicide treatments used to prevent crop production losses. We recently introduced a new phytoalexin-based method for a non-invasive detection of crop diseases using their fluorescence. The causal agent of grapevine downy mildew, Plasmopara viticola, induces the synthesis of stilbenoid phytoalexins by the host, Vitis vinifera, early upon infection. These stilbenoids emit violet-blue fluorescence under UV light. A hand-held solid-state UV-LED-based field fluorimeter, named Multiplex 330, was used to measure stilbenoid phytoalexins in a vineyard. It allowed us to non-destructively detect and monitor the naturally occurring downy mildew infections on leaves in the field.

• Authors: Gwendal Latouche, Christian Debord, Marc Raynal, Charlotte Milhade, Zoran G Cerovic
• Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 14 Issue 10 Pg. 1807-13 (Oct 2015) ISSN: 1474-9092 [Electronic] England
• PMID: 26293623 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Fluorometry (methods)
• Fungi, Unclassified (isolation & purification, physiology)
• Plant Diseases (microbiology)
• Time Factors
• Vitis (microbiology)