Abstract | BACKGROUND: METHODS: The aim of this study was to test the presence and diagnostic significance of anti-LRP4 autoantibodies in an Italian population of 101 myasthenic patients (55 dSN, 23 AChR positive and 23 MuSK positive), 45 healthy blood donors and 40 patients with other neurological diseases as controls. All sera were analyzed by a cell-based antigen assay employing LRP4-transfected HEK293T cells, along with a flow cytofluorimetric detection system. RESULTS: We found a 14.5% (8/55) frequency of positivity in the dSN-MG group and a 13% frequency of co-occurrence (3/23) in both AChR and MuSK positive patients; moreover, we report a younger female prevalence with a mild form of disease in LRP4-positive dSN-MG individuals. CONCLUSION: Our data confirm LRP4 as a new autoimmune target, supporting the value of including anti-LRP4 antibodies in further studies on Myasthenia gravis.
|
Authors | Mariapaola Marino, Flavia Scuderi, Daniela Samengo, Giorgia Saltelli, Maria Teresa Maiuri, Chengyong Shen, Lin Mei, Mario Sabatelli, Giovambattista Pani, Giovanni Antonini, Amelia Evoli, Emanuela Bartoccioni |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 8
Pg. e0135378
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 26284792
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Autoantibodies
- LDL-Receptor Related Proteins
- LRP4 protein, human
- Receptors, Cholinergic
- MUSK protein, human
- Receptor Protein-Tyrosine Kinases
|
Topics |
- Autoantibodies
(blood)
- Case-Control Studies
- Female
- Flow Cytometry
- HEK293 Cells
- Humans
- Immunoblotting
- Immunoprecipitation
- LDL-Receptor Related Proteins
(immunology, metabolism)
- Male
- Middle Aged
- Myasthenia Gravis
(blood, diagnosis, immunology)
- Receptor Protein-Tyrosine Kinases
(immunology, metabolism)
- Receptors, Cholinergic
(immunology, metabolism)
|