Abstract | PURPOSE: METHODS: Male CD1 strain mice were fed either a standard iron diet ( SID) or the same diet with extra iron added (HID) for either 3 months or 10 months. Mice were analyzed with immunofluorescence and Perls' histochemical iron stain to assess iron levels. Levels of ferritin, transferrin receptor, and oxidative stress gene mRNAs were measured by quantitative PCR (qPCR) in neural retina (NR) and isolated RPE. Morphology was assessed in plastic sections. RESULTS:
Ferritin immunoreactivity demonstrated a modest increase in the RPE in 10-month HID mice. Analysis by qPCR showed changes in mRNA levels of iron-responsive genes, indicating moderately increased iron in the RPE of 10-month HID mice. However, even by age 18 months, there was no Perls' signal in the retina or RPE and no retinal degeneration. CONCLUSIONS: These findings indicate that iron absorbed from the diet can modestly increase the level of iron deposition in the wild-type mouse RPE without causing RPE or retinal degeneration. This suggests regulation of retinal iron uptake at the blood-retinal barriers.
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Authors | Devang L Bhoiwala, Ying Song, Alyssa Cwanger, Esther Clark, Liang-liang Zhao, Chenguang Wang, Yafeng Li, Delu Song, Joshua L Dunaief |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 56
Issue 9
Pg. 5344-52
(Aug 2015)
ISSN: 1552-5783 [Electronic] United States |
PMID | 26275132
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Iron, Dietary
- Receptors, Transferrin
- RNA
- Iron
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Topics |
- Animals
- Disease Models, Animal
- Iron
(metabolism)
- Iron Overload
(chemically induced, genetics, metabolism)
- Iron, Dietary
(toxicity)
- Macular Degeneration
(genetics, metabolism, pathology)
- Male
- Mice
- Mice, Transgenic
- Oxidative Stress
- RNA
(genetics)
- Real-Time Polymerase Chain Reaction
- Receptors, Transferrin
(biosynthesis, genetics)
- Retinal Pigment Epithelium
(drug effects, metabolism, pathology)
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