Estimating Ixodes ricinus densities on the landscape scale.

Abstract

BACKGROUND:

The study describes the estimation of the spatial distribution of questing nymphal tick densities by investigating Ixodes ricinus in Southwest Germany as an example. The production of high-resolution maps of questing tick densities is an important key to quantify the risk of tick-borne diseases. Previous I. ricinus maps were based on quantitative as well as semi-quantitative categorisations of the tick density observed at study sites with different vegetation types or indices, all compiled on local scales. Here, a quantitative approach on the landscape scale is introduced.

METHODS:

During 2 years, 2013 and 2014, host-seeking ticks were collected each month at 25 sampling sites by flagging an area of 100 square meters. All tick stages were identified to species level to select nymphal ticks of I. ricinus, which were used to develop and calibrate Poisson regression models. The environmental variables height above sea level, temperature, relative humidity, saturation deficit and land cover classification were used as explanatory variables.

RESULTS:

The number of flagged nymphal tick densities range from zero (mountain site) to more than 1,000 nymphs/100 m(2). Calibrating the Poisson regression models with these nymphal densities results in an explained variance of 72 % and a prediction error of 110 nymphs/100 m(2) in 2013. Generally, nymphal densities (maximum 374 nymphs/100 m(2)), explained variance (46 %) and prediction error (61 nymphs/100 m(2)) were lower in 2014. The models were used to compile high-resolution maps with 0.5 km(2) grid size for the study region of the German federal state Baden-Württemberg. The accuracy of the mapped tick densities was investigated by leave-one-out cross-validation resulting in root-mean-square-errors of 227 nymphs/100 m(2) for 2013 and 104 nymphs/100 m(2) for 2014.

CONCLUSIONS:

The methodology introduced here may be applied to further tick species or extended to other study regions. Finally, the study is a first step towards the spatial estimation of tick-borne diseases in Central Europe.

• Authors: Denise Boehnke, Katharina Brugger, Miriam Pfäffle, Patrick Sebastian, Stefan Norra, Trevor Petney, Rainer Oehme, Nina Littwin, Karin Lebl, Johannes Raith, Melanie Walter, Reiner Gebhardt, Franz Rubel
• Journal: International journal of health geographics (Int J Health Geogr) Vol. 14 Pg. 23 (Aug 14 2015) ISSN: 1476-072X [Electronic] England
• PMID: 26272596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Environment
• Germany (epidemiology)
• Insect Vectors
• Ixodes (growth & development)
• Lyme Disease
• Poisson Distribution
• Population Density
• Tick Infestations (epidemiology)