Polyethylenimine-coated SPION exhibits potential intrinsic anti-metastatic properties inhibiting migration and invasion of pancreatic tumor cells.

Abstract

Due to its aggressive behavior, pancreatic cancer is one of the principal causes of cancer-related deaths. The highly metastatic potential of pancreatic tumor cells demands the development of more effective anti-metastatic approaches for this disease. Although polyethylenimine-coated superparamagnetic iron oxide nanoparticles (PEI-coated SPIONs) have been studied for their utility as transfection agents, little is known of their effect on tumor cell biology. Here we demonstrated that PEI-coated SPIONs have potent inhibitory effects on pancreatic tumor cell migration/invasion, through inhibition of Src kinase and decreased expression of MT1-MMP and MMP2 metalloproteinases. When treated with PEI-coated SPIONs, the pancreatic tumor cell line Pan02 showed reduced invadosome density and thus, a decrease in their ability to invade through basement membrane. These nanoparticles temporarily downmodulated microRNA-21, thereby upregulating the cell migration inhibitors PTEN, PDCD4 and Sprouty-1. PEI-coated SPIONs thus show intrinsic, possibly anti-metastatic properties for modulating pancreatic tumor cell migration machinery, which indicates their potential as anti-metastatic agents for treatment of pancreatic cancer.

• Authors: Vladimir Mulens-Arias, José Manuel Rojas, Sonia Pérez-Yagüe, María del Puerto Morales, Domingo F Barber
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 216 Pg. 78-92 (Oct 28 2015) ISSN: 1873-4995 [Electronic] Netherlands
• PMID: 26264831 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Ferric Compounds
• MIRN21 microRNA, human
• Matrix Metalloproteinase Inhibitors
• MicroRNAs
• ferric oxide
• Polyethyleneimine

Topics

• Cell Line, Tumor
• Cell Movement (drug effects, genetics)
• Cell Proliferation (drug effects)
• Ferric Compounds (chemistry, therapeutic use)
• Gene Transfer Techniques
• HEK293 Cells
• Humans
• Magnetics
• Matrix Metalloproteinase Inhibitors (pharmacology)
• MicroRNAs
• Nanoparticles
• Neoplasm Invasiveness
• Neoplasm Metastasis (therapy)
• Pancreatic Neoplasms (drug therapy)
• Polyethyleneimine (chemistry)
• Signal Transduction (drug effects)
• Transfection