[Establishment and diagnostic performance of biotin-avidin complex enzyme linked immunosorbent assay of detecting specific IgG4 of clonorchiasis].

Abstract

OBJECTIVE:

To explore the performance of the biotin-avidin complex enzyme linked immunosorbent assay of detecting specific IgG4 for the diagnosis of clonorchiasis.

METHODS:

The avidin-biotin complex enzyme linked immunosorbent assay of detecting specific IgG4 (IgG4-ABC-ELISA)against Clonorchis sinensis was established, and used to detect the serum samples of patients with clonorchiosis sinensis, schistosomiasis japonica, paragonimiasis, toxoplasmosis, echinococcosis, cysticercosis and sparganosis mansoni. At the same time, these sera were analyzed by the ELISA of detecting IgG4 (IgG4-ELISA) and ELISA of detecting the total IgG (IgG-ELISA) as controls. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the respective diagnostic performance of the three methods were compared.

RESULTS:

The IgG4-ABC-ELISA for diagnosis of clonorchiasis was established successfully. The sensitivity and specificity of the IgG4-ABC-ELISA for detecting clonorchiasis were 90.0% and 98.2% respectively, and PPV and NPV were 93.8% and 97.0% respectively. Its diagnostic performance was 96.3%. The sensitivity and specificity of the IgG4-ELISA for detecting clonorchiasis were 86.0% and 98.2% respectively, and PPV and NPV were 93.5% and 95.9% respectively. Its diagnostic performance was 95.4%. The sensitivity and specificity of the IgG-ELISA for detecting clonorchiasis were 94.0% and 88.1% respectively, and PPV and NPV were 70.1% and 98.0% respectively. Its diagnostic performance was 89.4%. The sensitivity of IgG4-ABC-ELISA was higher than that of IgG4-ELISA (P < 0.05), and the specificity of IgG4-ABC-ELISA was higher than that of IgG-ELISA (P < 0.05).

CONCLUSIONS:

IgG4-ABC-ELISA of detecting specific antibody IgG4 against Clonorchis sinensis has high sensitivity and specificity. Therefore, it has a good application value in the diagnosis of clonorchiasis.

• Authors: Jie Wang, Li-jun Song, Chuan-xin Yu, Shuang Shen, Yong-liang Xu, Xu-ren Yin, Xue-dan Ke, Hong Gao, Qian Liu
• Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control (Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi) Vol. 27 Issue 2 Pg. 156-61 (Apr 2015) ISSN: 1005-6661 [Print] China
• PMID: 26263776 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Immunoglobulin G
• Avidin
• Biotin

Topics

• Antibodies, Helminth (blood)
• Avidin
• Biotin
• Clonorchiasis (diagnosis)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin G (blood)
• Sensitivity and Specificity