Piperine is an exact of the active phenolic component from Black pepper. It has been reported to have many
biological activities including
anti-oxidant, anti-inflammatory and anti-
tumor effects.
Intervertebral disc degeneration (IDD) is a degenerative disease closely relate to
inflammation of nucleus pulposus (NP) cells. This study aimed to assess the anti-inflammatory and anti-catabolic effects of
piperine in rat intervertebral disc using in vitro and ex vivo analyzes. We demonstrated that
piperine could inhibit LPS induced expression and production of inflammatory factors and catabolic
proteases in NP cells culture model. It significantly inhibited multiple inflammatory factors and oxidative stress-associated genes (IL-1β, TNF-α, IL-6, iNOS),
MMPs (MMP-3, MMP-13), ADAMTS (ADAMTS-4, ADAMTS-5)
mRNA expression and NO production in a concentration-dependent manner. Moreover,
piperine could reverse the LPS-induced inhibition of gene expression of
aggrecan and
collagen-II. Histologic and
dimethylmethylene blue analysis indicated
piperine could also against LPS induced
proteoglycan (PG) depletion in a rat intervertebral disc culture model. Western blot results showed that
piperine inhibited the LPS-mediated phosphorylation of JNK and activation of NF-κB. Finally, our results demonstrated the ability of
piperine to antagonize LPS-mediated
inflammation of NP cells and suppression of PG in rat intervertebral disc, suggesting a potential agent for treatment of IDD in future.