HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis.

Abstract
The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.
AuthorsBenedikt Nimmervoll, Lilia A Chtcheglova, Kata Juhasz, Nunilo Cremades, Francesco A Aprile, Alois Sonnleitner, Peter Hinterdorfer, Laszlo Vigh, Johannes Preiner, Zsolt Balogi
JournalFEBS letters (FEBS Lett) Vol. 589 Issue 19 Pt B Pg. 2747-53 (Sep 14 2015) ISSN: 1873-3468 [Electronic] England
PMID26257049 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Chemical References
  • Clathrin
  • HSP70 Heat-Shock Proteins
  • Protein Aggregates
Topics
  • Animals
  • Cell Line, Tumor
  • Clathrin (metabolism)
  • Endocytosis
  • HSP70 Heat-Shock Proteins (chemistry, metabolism)
  • Melanoma, Experimental (metabolism, pathology)
  • Membrane Microdomains
  • Mice
  • Protein Aggregates

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: