A Catalytic DNA Probe with Stem-loop Motif for Human T47D Breast Cancer Cells.

Abstract

In vitro selection methods allow for isolation of DNAzymes (catalytic DNAs) from random DNA pools. Here we describe a fluorogenic DNAzyme, LYF5, isolated using a double-random selection approach: a random DNA pool was selected against a complex molecular mixture derived from a breast cancer cell line, T47D. LYF5 specifically indicates the T47D breast cancer cell line with high sensitivity. After sequence optimization, the second-generation DNAzyme, 2G-LYF5, exhibited an approximately 2-fold higher cleavage percentage. Finally, we have determined that the intramolecular stem-loop motif plays a crucial role in 2G-LYF5 activity. Our findings underscore the capability of single-stranded DNA molecules to perform highly sophisticated functions that are amenable to the development of diagnostic tests for early identification of breast cancer.

• Authors: Fei Gao, Feng Liu, Jing Zheng, MeiYun Zeng, Yuyang Jiang
• Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (Anal Sci) Vol. 31 Issue 8 Pg. 815-22 ( 2015) ISSN: 1348-2246 [Electronic] Switzerland
• PMID: 26256606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes
• DNA, Catalytic

Topics

• Base Sequence
• Breast Neoplasms (pathology)
• Cell Line, Tumor
• DNA Probes (genetics, metabolism)
• DNA, Catalytic (genetics, metabolism)
• Humans
• Inverted Repeat Sequences
• Molecular Sequence Data