Rhamnogalacturonan I containing homogalacturonan inhibits colon cancer cell proliferation by decreasing ICAM1 expression.

Abstract

Pectin modified with pH, heat or enzymes, has previously been shown to exhibit anti-cancer activity. However, the structural requirements for modified pectin bioactivity have rarely been addressed. In this study several pectin extracts representing different structural components of pectin were assessed for effects against colon cancer cells. Rhamnogalacturonan I (RGI) extracts reduced proliferation of DLD1 and HCT116 colon cancer cells in a dose- and time-dependent manner. RGI reduced ICAM1 gene expression and siRNA-mediated knockdown of ICAM1 expression decreased cell proliferation providing a potential novel mechanism for the anti-cancer activity of pectin. Structural analysis of bioactive and non-bioactive RGIs suggested that a homogalacturonan component is maybe essential for the anti-proliferative activity, furthering the understanding of the structural requirements for pectin bioactivity.

• Authors: Ellen G Maxwell, Ian J Colquhoun, Hoa K Chau, Arland T Hotchkiss, Keith W Waldron, Victor J Morris, Nigel J Belshaw
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 132 Pg. 546-53 (Nov 05 2015) ISSN: 1879-1344 [Electronic] England
• PMID: 26256381 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• RNA, Small Interfering
• rhamnogalacturonan I
• Intercellular Adhesion Molecule-1
• Pectins
• polygalacturonic acid

Topics

• Antineoplastic Agents (chemistry, toxicity)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Colonic Neoplasms (metabolism, pathology)
• HCT116 Cells
• Humans
• Intercellular Adhesion Molecule-1 (chemistry, genetics, metabolism)
• Magnetic Resonance Spectroscopy
• Pectins (chemistry, toxicity)
• RNA Interference
• RNA, Small Interfering (metabolism)