Heme oxygenase (HO)-1 confers transient resistance against oxidative damage, including renal
ischemia-reperfusion injury (IRI). We investigated the potential protective effect of HO-1 induction in a mouse model of renal IRI induced by bilateral clamping of the kidney arteries. The mice were randomly assigned to five groups to receive an
intraperitoneal injection of PBS,
hemin (an HO-1 inducer, 100μmol/kg), hemin+ZnPP (an HO-1 inhibitor, 5mg/kg), hemin+PD98059 (a
MEK-ERK inhibitor, 10mg/kg) or a
sham operation. All of the groups except for the
sham-operated group underwent 25min of
ischemia and 24 to 72h of reperfusion. Renal function and tubular damage were assessed in the mice that received
hemin or the vehicle treatment prior to IRI. The renal injury score and HO-1
protein levels were evaluated via H&E and immunohistochemistry staining.
Hemin-preconditioned mice exhibited preserved renal cell function (BUN: 40±2mg/dl,
creatinine: 0.53±0.06mg/dl), and the tubular injury score at 72h (1.65±0.12) indicated that tubular damage was prevented. Induction of HO-1 induced the phosphorylation of
extracellular signal-regulated kinases (ERK) 1/2. However, these effects were abolished with ZnPP treatment. Kidney function (BUN: 176±49mg/dl,
creatinine: 1.54±0.39mg/dl) increased, and the tubular injury score (3.73±0.09) indicated that tubular damage also increased with ZnPP treatment. HO-1-induced tubular epithelial proliferation was attenuated by
PD98059. Our findings suggest that HO-1 preconditioning promotes ERK1/2 phosphorylation and enhances tubular recovery, which subsequently prevents further renal injury.