Abstract | AIMS: MAIN METHODS: The expression of erythropoietin and its receptor in MSN and HEK 293 was analysed by RT-PCR, immunocytochemistry, and Western blotting. The effect of erythropoietin on cell viability and proliferation was evaluated by the MTT assay, and by the Click-iT EdU Alexa Fluor 647 kit, respectively. For the cyto-protective assays, cells were incubated with erythropoietin before etoposide and vincristine treatment. Activation of signalling pathways was studied by Western blotting. KEY FINDINGS: SIGNIFICANCE: These results indicate that erythropoietin induces proliferation of MSN cells, and that it can ameliorate vincristine- and etoposide-induced apoptosis of these cells. Erythropoietin-mediated neuroprotection was regulated by the combined effect of the ERK1/2 and AKT signalling pathways. Our findings provide further insights into the potential effect of erythropoietin on neuroblastoma cells.
|
Authors | Maria Jose Vazquez-Mellado, Cecilia Aguilar, Leticia Rocha-Zavaleta |
Journal | Life sciences
(Life Sci)
Vol. 137
Pg. 142-9
(Sep 15 2015)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 26232556
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Protective Agents
- Receptors, Erythropoietin
- Erythropoietin
- Vincristine
- Etoposide
- Proto-Oncogene Proteins c-akt
- Extracellular Signal-Regulated MAP Kinases
|
Topics |
- Cell Death
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Erythropoietin
(biosynthesis, pharmacology)
- Etoposide
(adverse effects)
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- HEK293 Cells
- Humans
- Kidney
(cytology, drug effects)
- Neuroblastoma
(enzymology, metabolism, pathology)
- Protective Agents
(pharmacology)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Receptors, Erythropoietin
(biosynthesis)
- Signal Transduction
(drug effects)
- Vincristine
(adverse effects)
|