Abstract | BACKGROUND: MATERIALS AND METHODS: CYP-17 gene polymorphisms (MspA1) were genotyped with PCR-RFLP in cancer patients (n=96), stone patients (n=102), cancer+stone patients (n=52) and age/sex matched control subjects (n=256). Lipid profile was estimated using a commercial kit and serum estrogen was measured using ELISA. RESULTS: The majority of the patients in all groups were females. The lipid profile and estrogen level were significantly higher among the study as compared to control groups. The frequency of mutant allele A2 of CYP17 MspA1 gene polymorphism was higher among cancer (OR=5.13, 95% CI+3.10-8.51, p=0.0001), stone (OR=5.69, 95%CI=3.46-9.37, p=0.0001) and cancer+stone (OR=3.54, 95%CI=1.90-6.60, p=0.0001) when compared with the control group. However there was no significant association between genotypes of CYP17 MspA1 gene polymorphism and circulating serum level of estrogen and lipid profile. CONCLUSIONS: A higher frequency of mutant genotype A1A2 as well as mutant allele A2 of CYP-17 gene polymorphism is significantly associated with risk of gallbladder cancer and stones. Elevated levels of estrogen and an altered lipid profile can be used as predictors ofgall bladder stones and cancer in post menopausal females in India.
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Authors | Shipra Dwivedi, Sarita Agrawal, Shraddha Singh, Amit Kumar Madeshiya, Devendra Singh, Abbas Ali Mahdi, Abhjeet Chandra |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 16
Issue 13
Pg. 5557-63
( 2015)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 26225710
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Steroid 17-alpha-Hydroxylase
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Topics |
- Adolescent
- Adult
- Aged
- Case-Control Studies
- Female
- Follow-Up Studies
- Gallbladder Neoplasms
(genetics, mortality, pathology)
- Gallstones
(genetics, mortality, pathology)
- Genotype
- Humans
- Male
- Middle Aged
- Neoplasm Staging
- Polymerase Chain Reaction
- Polymorphism, Genetic
(genetics)
- Polymorphism, Restriction Fragment Length
- Prognosis
- Risk Factors
- Steroid 17-alpha-Hydroxylase
(genetics)
- Survival Rate
- Urinary Bladder Calculi
(genetics, mortality, pathology)
- Young Adult
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