Abstract | AIMS: MAIN METHODS: A suitable sepsis mouse model was first established using lipopolysaccharide (LPS) injected intraperitoneally (i.p.). Q or Q3G was administered i.p. to septic mice in a prophylactic or therapeutic manner. Pro-inflammatory (TNF-α, IL-1β and IL-6) and anti-inflammatory (IL-10) cytokine secretion profiles by peritoneal macrophages of the mice were measured using ELISA. KEY FINDINGS: Mice which received 8mg/kg BW LPS i.p. for 12h resulted in intermediate systemic inflammation, suggesting a useful mild septic mouse model. At non-toxic doses, Q or Q3G (0.06 or 0.15μmol/mouse) i.p. injected in a prophylactic manner significantly (P<0.05) increased anti-inflammatory IL-10 secretions by peritoneal macrophages of the LPS-induced septic mice. Q, but not Q3G, i.p. injected in a therapeutic manner significantly (P<0.05) increased IL-10 secretions by peritoneal macrophages of the septic mice. SIGNIFICANCE: Our data suggest that Q, but not Q3G, has pharmacological effects to ameliorate systemic inflammation. These results are the first to show that Q has potent potential against sepsis in both prophylactic and therapeutic manners in vivo.
|
Authors | Yi-Ru Liao, Jin-Yuarn Lin |
Journal | Life sciences
(Life Sci)
Vol. 137
Pg. 89-97
(Sep 15 2015)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 26209141
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Inflammation Mediators
- Lipopolysaccharides
- Quercetin
|
Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage, pharmacology, therapeutic use)
- Cells, Cultured
- Dose-Response Relationship, Drug
- Female
- Inflammation
(complications, drug therapy, immunology, prevention & control)
- Inflammation Mediators
(metabolism)
- Injections, Intraperitoneal
- Lipopolysaccharides
(immunology)
- Macrophages
(drug effects, metabolism)
- Mice
- Quercetin
(administration & dosage, analogs & derivatives, pharmacology, therapeutic use)
- Sepsis
(complications, metabolism)
|