Abstract |
The infection routes of HIV-1 can affect several viral properties, including dissemination, pathogenesis, and immune evasion. In this study, we evaluated the inhibitory activity of a wide variety of anti-HIV drugs, focusing on the impact that different infection pathways have on their efficacy. Compared to cell-free infection, inhibitory activities were reduced in cell-to-cell productive transmission for all drugs tested. We detected weak reporter-expressing target cells after cell-to-cell transmission in the presence of integrase strand transfer inhibitors (INSTIs). Further analysis revealed that this expression was mainly due to unintegrated circular HIV (cHIV) DNAs, consisting of 1-LTR and 2-LTR circles. When in vitro-constructed cHIV DNAs were introduced into cells, the production of infectious and intercellular transmittable virions was observed, suggesting that cHIV DNA could be a source of infectious virus. These results highlight some advantages of the cell-to-cell infection mode for viral expansion, particularly in the presence of anti-retroviral drugs.
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Authors | Kazuya Shimura, Paola Miyazato, Shinya Oishi, Nobutaka Fujii, Masao Matsuoka |
Journal | Virology
(Virology)
Vol. 484
Pg. 364-376
(Oct 2015)
ISSN: 1096-0341 [Electronic] United States |
PMID | 26186575
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
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Topics |
- Anti-HIV Agents
(pharmacology)
- Cell Line
- HIV-1
(drug effects, physiology)
- Humans
- Virus Internalization
(drug effects)
- Virus Release
(drug effects)
- Virus Replication
(drug effects)
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