Oxidative stress-induced C/EBPβ inhibits β-catenin signaling molecule involving in the pathology of preeclampsia.

Abstract

INTRODUCTION:

Oxidative stress-induced trophoblast cell dysfunction is a major pathology in preeclampsia (PE). Recently, CCAAT/enhancer binding protein beta (C/EBPβ) has been investigated as a tumor suppressor that participates in tumor invasion. However, the function of C/EBPβ in trophoblast cells remains unknown. Our study was designed to detect the expression of C/EBPβ in the preeclamptic placenta and to identify the underlying mechanisms of oxidative stress.

METHODS:

Human placental tissues with PE were collected. The expression of C/EBPβ and β-catenin were detected. Human first trimester extravillous trophoblast cell (HTR8/SVneo) line exposed to hypoxia/reoxygenation (H/R) was employed as an oxidative stress model in vitro to investigate the effects of C/EBPβ on invasion and the expression of β-catenin. Moreover, first trimester-derived placental villous explants were used to verify the effects of C/EBPβ and β-catenin in placentation.

RESULTS:

In preeclamptic placentas, C/EBPβ was overexpressed and β-catenin was decreased. In addition, C/EBPβ was found to have increased expression in H/R-treated HTR8/SVneo cells and villous explants. C/EBPβ knockdown and β-catenin activation could significantly promote the invasion of HTR8/SVneo cells, enhance the outgrowth and migration in villous explants and inhibit the excessive generation of intracellular ROS. These findings might be related to the increased activities of MMP-2/9 and the decreased expression of TIMP-1/2. Meanwhile, C/EBPβ knockdown remarkably increased the expression of β-catenin.

DISCUSSION:

We hypothesize that the oxidative stress-induced overexpression of C/EBPβ might influence the activity of MMPs by regulating the Wnt/β-catenin signaling pathway to affect the invasion of trophoblast cells, which then participate in the pathogenesis of preeclampsia.

• Authors: B Zhuang, X Luo, H Rao, Q Li, N Shan, X Liu, H Qi
• Journal: Placenta (Placenta) Vol. 36 Issue 8 Pg. 839-46 (Aug 2015) ISSN: 1532-3102 [Electronic] Netherlands
• PMID: 26166436 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• CCAAT-Enhancer-Binding Protein-beta
• Reactive Oxygen Species
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• beta Catenin
• Matrix Metalloproteinases

Topics

• CCAAT-Enhancer-Binding Protein-beta (metabolism)
• Cell Line
• Female
• Humans
• Hypoxia (metabolism)
• Matrix Metalloproteinases (metabolism)
• Oxidative Stress (physiology)
• Placenta (metabolism)
• Placentation (physiology)
• Pre-Eclampsia (metabolism)
• Pregnancy
• Reactive Oxygen Species (metabolism)
• Signal Transduction (physiology)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)
• beta Catenin (metabolism)