Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green.

Abstract

We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.

• Authors: Hiroki Ueo, Yoshiaki Shinden, Taro Tobo, Ayako Gamachi, Mitsuaki Udo, Hisateru Komatsu, Sho Nambara, Tomoko Saito, Masami Ueda, Hidenari Hirata, Shotaro Sakimura, Yuki Takano, Ryutaro Uchi, Junji Kurashige, Sayuri Akiyoshi, Tomohiro Iguchi, Hidetoshi Eguchi, Keishi Sugimachi, Yoko Kubota, Yuichiro Kai, Kenji Shibuta, Yuko Kijima, Heiji Yoshinaka, Shoji Natsugoe, Masaki Mori, Yoshihiko Maehara, Masayo Sakabe, Mako Kamiya, John W Kakareka, Thomas J Pohida, Peter L Choyke, Hisataka Kobayashi, Hiroaki Ueo, Yasuteru Urano, Koshi Mimori
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 12080 (Jul 13 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26165706 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• Rhodamines
• gamma-Glutamyltransferase

Topics

• Breast (metabolism, pathology)
• Breast Neoplasms (diagnosis, metabolism, pathology)
• Cell Line, Tumor
• Female
• Fluorescent Dyes (metabolism)
• Humans
• MCF-7 Cells
• Rhodamines (metabolism)
• Sensitivity and Specificity
• gamma-Glutamyltransferase (metabolism)