Abstract | BACKGROUND: Mesenchymal stem cells (MSCs) have the potential to differentiate into multiple cell lineages. Given this potential for tissue regeneration, MSC-based therapeutic applications have been considered in recent years. However, ischemia-induced apoptosis has been reported to be one of the main causes of MSC death following transplantation. The primary objective of this study was to determine whether a natural antioxidant, fucoidan, could protect MSCs from ischemia-induced apoptosis in vitro and in vivo. Furthermore, we investigated the mechanism of action of fucoidan's anti-ischemic effect in MSCs. METHODS AND RESULT: CONCLUSION: Taken together, these results show that fucoidan protects MSCs from ischemia-induced cell death by modulation of apoptosis-associated proteins and cellular ROS levels through regulation of the MnSOD and Akt pathways, suggesting that fucoidan could be powerful therapeutic adjuvant for MSC-based therapy in ischemic diseases.
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Authors | Yong-Seok Han, Jun Hee Lee, Jin Sup Jung, Hyunjin Noh, Moo Jun Baek, Jung Min Ryu, Yeo Min Yoon, Ho Jae Han, Sang Hun Lee |
Journal | International journal of cardiology
(Int J Cardiol)
Vol. 198
Pg. 187-95
(Nov 01 2015)
ISSN: 1874-1754 [Electronic] Netherlands |
PMID | 26163916
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Cell Survival
(drug effects, physiology)
- Dose-Response Relationship, Drug
- Hindlimb
(blood supply)
- Humans
- Ischemia
(drug therapy, pathology)
- Mesenchymal Stem Cell Transplantation
(methods)
- Mesenchymal Stem Cells
(drug effects, physiology)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neovascularization, Physiologic
(drug effects, physiology)
- Oxidative Stress
(drug effects, physiology)
- Polysaccharides
(pharmacology, therapeutic use)
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