Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.

Abstract

OBJECTIVE:

This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.

METHODS:

Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.

RESULTS:

In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.

CONCLUSION:

These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

• Authors: Young Seok Lee, Min Jung Park, Sea Hee Park, Ja Seong Koo, Hwa Sook Moon, Bo Sun Joo
• Journal: Clinical and experimental reproductive medicine (Clin Exp Reprod Med) Vol. 42 Issue 2 Pg. 51-7 (Jun 2015) ISSN: 2233-8233 [Print] Korea (South)
• PMID: 26161333 (Publication Type: Journal Article)