Marine n-3 PUFA protects hearts from I/R injury via restoration of mitochondrial function.

Abstract

OBJECTIVES:

Overwhelming evidence shows that dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs) elicits protective effects on patients with cardiovascular disease. However, the detailed mechanisms underlying n-3 PUFA-mediated cardioprotection are unknown, and examined in the present study.

METHODS:

We evaluated heart performances with Langendorff perfusion apparatus. Meanwhile, whole mitochondria were purified from non-perfused hearts for functional assessment, and lipid peroxidation level was measured as well.

RESULTS:

Compared with control groups, hearts from n-3 PUFA-supplemented rats showed improved functional recovery and reduced tissue injury following ischemia/reperfusion (I/R). Furthermore, the mitochondrial function of PUFA-treated hearts was significantly enhanced, as demonstrated by biochemical analysis of respiratory chain activity. In addition, thiobarbituric acid-reactive substance or TBARS assay revealed that lipid peroxidation product, malondialdehyde or MDA, in the mitochondria was significantly reduced by PUFA treatment.

CONCLUSION:

Taken together, our data indicate that marine n-3 PUFA could improve cardiac performance after I/R injury by restoring mitochondrial respiratory activities and attenuating lipid peroxidation.

• Authors: Kang Gao, Liwei Chen, Ming Yang, Ling Han, Sun Yiguang, Han Zhao, Xin Chen, Wenze Hu, Haifeng Liang, Jingguang Luo, Jie Ma
• Journal: Scandinavian cardiovascular journal : SCJ (Scand Cardiovasc J) Vol. 49 Issue 5 Pg. 264-9 ( 2015) ISSN: 1651-2006 [Electronic] England
• PMID: 26159250 (Publication Type: Journal Article)

Chemical References

• Fatty Acids, Omega-3
• Fish Oils

Topics

• Animals
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Fatty Acids, Omega-3 (pharmacology, therapeutic use)
• Fish Oils (pharmacology, therapeutic use)
• In Vitro Techniques
• Lipid Peroxidation (drug effects)
• Male
• Mitochondria, Heart (drug effects)
• Myocardial Contraction (drug effects)
• Myocardial Reperfusion Injury (prevention & control)
• Rats, Sprague-Dawley