Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension.

Abstract

The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to selected clinical parameters. The group of 26 patients [AdipoR(-)] showed lower and the group of 31 patients [AdipoR(+)] showed higher AdipoRs protein expression than the control and each other (P < 0.01 for neutrophils, P < 0.05 for monocytes). The AdipoR(+) leukocytes expressed higher AdipoR1 mRNA levels (RT-PCR) than AdipoR(-) ones and controls (P = 0.022 and P = 0.007, resp.). Despite greater BMI, the AdipoR(-) patients had unchanged serum adiponectin levels. In contrast, AdipoR(+) patients had lower serum adiponectin concentrations than the AdipoR(-) ones and controls (P < 0.001). The AdipoR(+) patients had higher blood pressure (P = 0.042) and greater carotid intima-media thickness (P = 0.017) than the AdipoR(-) ones. The stage of hypertension was associated with increased neutrophil but not monocyte AdipoR1 density (AdipoR1 MFI) (P < 0.05). Severe ambulatory hypertension was presented more often in AdipoR(+) patients than in AdipoR(-) ones (51.6% versus 26.9%, resp.; P < 0.01). In conclusion, neutrophil AdipoRs upregulation was associated with early stages of vascular injury, hypertension severity, and low serum levels of adiponectin.

• Authors: Lidia Gackowska, Mieczyslaw Litwin, Joanna Trojanek, Andrzej Eljaszewicz, Izabela Kubiszewska, Anna Niemirska, Aldona Wierzbicka, Jacek Michalkiewicz
• Journal: BioMed research international (Biomed Res Int) Vol. 2015 Pg. 742646 ( 2015) ISSN: 2314-6141 [Electronic] United States
• PMID: 26146630 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ADIPOQ protein, human
• ADIPOR1 protein, human
• ADIPOR2 protein, human
• Adiponectin
• RNA, Messenger
• Receptors, Adiponectin

Topics

• Adiponectin (blood)
• Adolescent
• Child
• Essential Hypertension
• Female
• Gene Expression Regulation
• Humans
• Hypertension (blood, genetics, pathology)
• Insulin Resistance (genetics)
• Leukocytes (metabolism, pathology)
• Male
• Neutrophils (metabolism, pathology)
• RNA, Messenger (blood)
• Receptors, Adiponectin (blood, genetics)