Evaluation of the R-Biopharm RIDA®GENE Panton-Valentine leukocidin (PVL) kit for the detection of Staphylococcus aureus PVL from pus samples.

Abstract

Staphylococcus aureus Panton-Valentine leukocidin (PVL) is associated with primary skin and soft-tissue infections (SSTI). We aimed to divert the RIDA®GENE PVL kit (RBiopharm) from its intended use on cultures to the detection of PVL-encoding genes directly from pus samples. Performance was compared with that of the in-house PCR method developed by the French National Reference Centre for Staphylococci. From June 2013 to May 2014, pus samples from S. aureus SSTI were tested. Our in-house PCR was performed on parallel cultures as the gold standard, while the RIDA®GENE PVL assay was used directly on pus samples from the sterile container, or a swab or an Eswab previously dipped in the pus. The kit specificity was also evaluated with pus samples that grew Streptococcus pyogenes. S. aureus reference strains harboring PVL-encoding genes, including known polymorphisms, were also tested. A total of 56 S. aureus-containing pus samples (28 PVL + and 28 PVL-) were collected and analyzed. Sensitivity and specificity of the commercial kit were 96.4 % and 100 % respectively, with equal performance whether tested directly from the sterile container or the Eswab. Sensitivity was lower (67.9 %) when the test was performed from a regular SSTI swab. None of the Streptococcus pyogenes pus samples scored positive (n = 5). Specificity was assessed using reference strains (n = 14); in all strains the PVL gene was correctly detected. This study identified the RIDA®GENE PVL kit as an efficient, sensitive, and specific tool for the rapid detection of PVL-encoding genes in pus samples.

• Authors: C Bouchiat, M Bes, C Bouveyron, F Vandenesch, A Tristan
• Journal: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (Eur J Clin Microbiol Infect Dis) Vol. 34 Issue 9 Pg. 1905-8 (Sep 2015) ISSN: 1435-4373 [Electronic] Germany
• PMID: 26139561 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• Exotoxins
• Leukocidins
• Panton-Valentine leukocidin

Topics

• Bacterial Toxins (analysis, genetics)
• Exotoxins (analysis, genetics)
• Humans
• Leukocidins (analysis, genetics)
• Molecular Diagnostic Techniques (methods)
• Real-Time Polymerase Chain Reaction (methods)
• Soft Tissue Infections (diagnosis, microbiology)
• Staphylococcal Skin Infections (diagnosis, microbiology)
• Staphylococcus aureus (genetics, metabolism)
• Streptococcus pyogenes (genetics, metabolism)
• Suppuration (microbiology)