Abstract | BACKGROUND: OBJECTIVE: METHODS: RESULTS: The ROS caused extensive alterations in conformation and function of tyrosinase. Protein-A purified IgGs from vitiligo patients (Vt- IgG) showed strong binding to ROS- tyrosinase in comparison with IgGs from healthy controls (p<0.001). Interestingly, not only was there an increased number of subjects positive for anti-ROS- tyrosinase-IgGs, but also the levels of these IgGs were significantly higher among vitiligo patients, whose DD were ≥10 years as compared to patients with short DD (<10 years). In addition, a significant correlation was observed between the levels of anti-ROS- tyrosinase-IgGs and the patients' ages or with disease severity. Experimentally induced anti-ROS- tyrosinase-IgGs show reactivity with tyrosinase from vitiligo patients. Furthermore, vitiligo patients had lower levels of tyrosinase activity compared with healthy controls. Not only these, levels of carbonylation were also higher among vitiligo patients whose DD were ≥10 years as compared to patients with DD<10 years. CONCLUSIONS: This is the first study to demonstrate the role of oxidized tyrosinase in vitiligo. Our novel results support an association between oxidized tyrosinase and vitiligo autoimmunity. The stronger antibodies response to oxidized tyrosinase in vitiligo patients with higher DD or with severe patients suggests that oxidized tyrosinase may be a useful biomarker in evaluating the progression of vitiligo and in elucidating the mechanisms of disease pathogenesis.
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Authors | Hani A Al-Shobaili, Zafar Rasheed |
Journal | Journal of dermatological science
(J Dermatol Sci)
Vol. 79
Issue 3
Pg. 203-13
(Sep 2015)
ISSN: 1873-569X [Electronic] Netherlands |
PMID | 26137927
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Autoantibodies
- Immunoglobulin G
- Reactive Oxygen Species
- Monophenol Monooxygenase
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Topics |
- Adolescent
- Adult
- Autoantibodies
(immunology)
- Case-Control Studies
- Child
- Female
- Humans
- Immunoglobulin G
(blood, immunology)
- Male
- Monophenol Monooxygenase
(drug effects, immunology, metabolism)
- Oxidation-Reduction
- Protein Carbonylation
- Reactive Oxygen Species
(pharmacology)
- Severity of Illness Index
- Time Factors
- Vitiligo
(enzymology, immunology)
- Young Adult
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