HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.

Abstract

A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots. Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis. The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %. Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR). The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group. PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group. The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla. As a result, AR possessed a similar immunoregulation function as NR.

• Authors: Juan Wang, Jing Li, Hongfa Li, Xiaolei Wu, Wenyuan Gao
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 177 Issue 1 Pg. 63-75 (Sep 2015) ISSN: 1559-0291 [Electronic] United States
• PMID: 26137876 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biological Products
• Peptides, Cyclic
• Polysaccharides
• Saponins

Topics

• Animals
• Batch Cell Culture Techniques (methods)
• Biological Products (immunology)
• Bioreactors
• Biosynthetic Pathways
• Caryophyllaceae (chemistry)
• Chromatography, High Pressure Liquid (methods)
• Female
• Hypersensitivity, Delayed (pathology)
• Mice
• Peptides, Cyclic (metabolism)
• Plant Roots (chemistry)
• Polysaccharides (pharmacology)
• Saponins (pharmacology)
• Spectrometry, Mass, Electrospray Ionization (methods)