Proteomic profiling of posterior longitudinal ligament of cervical spine.

Abstract

OBJECTIVE:

To identify putative biomarkers for ossification of posterior longitudinal ligament (OPLL).

MATERIAL AND METHODS:

Proteomic analysis was performed in 4 ligament samples from OPLL patients and healthy controls. RT-PCR was used to further verify the proteomic analysis results.

RESULTS:

A total of 50 differentially expressed spots were detected in 2-D electrophoresis between the two groups. In protein/peptide analysis, 21 proteins or peptides were finally identified. Besides 13 hematic proteins and 2 unknown proteins, 6 other proteins were differentially expressed. Among them, carbonic anhydrase I, NAD(P) dependent steroid dehydrogenase-like, billiverdin reductase B and alpha-1 collagen VI were down-regulated, while osteoglycin and nebulin-related anchoring protein were up-regulated. The results of NAD(P) dependent steroid dehydrogenase-like, alpha-1 collagen VI and nebulin-related anchoring protein were validated by RT-PCR.

CONCLUSION:

These differentially expressed proteins could play a role in the onset and progression of OPLL.

• Authors: Ying Zhang, Baifeng Liu, Jiang Shao, Jia Song, Jing Zhang
• Journal: International journal of clinical and experimental medicine (Int J Clin Exp Med) Vol. 8 Issue 4 Pg. 5631-9 ( 2015) ISSN: 1940-5901 [Print] United States
• PMID: 26131146 (Publication Type: Journal Article)