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Fyn arrests swainsonine-induced apoptosis in 293T cells via Akt and its phosphorylation.

Abstract
Swainsonine (SW), an extract from Astragalus membranaceus, represents a new class of compounds that inhibit growth and induce apoptosis in a cancer model. In this study, we demonstrated the effect of Fyn on SW-induced apoptosis in 293T cells. Western blotting was used to measure the expression of the apoptosis-related factors caspase-3, Bcl-2, Bax, and the key factor Akt (also known as protein kinase B). Apoptosis increased dramatically after treatment with SW. Unlike the control group, after transfection with Fyn, the expression of Bcl-2, in contrast to Bax, was markedly upregulated. The results also showed that the protein expression levels of Akt and phosphorylated Akt were markedly increased. Our results establish that Fyn can arrest SW-induced apoptosis via the activity of Akt and its effective phosphorylation in 293T cells.
AuthorsL An, W W Li, G C Cheng
JournalGenetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 2 Pg. 5304-9 (May 18 2015) ISSN: 1676-5680 [Electronic] Brazil
PMID26125726 (Publication Type: Journal Article)
Chemical References
  • Plant Extracts
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • FYN protein, human
  • Proto-Oncogene Proteins c-fyn
  • Oncogene Protein v-akt
  • Swainsonine
Topics
  • Apoptosis (drug effects)
  • Astragalus propinquus (chemistry)
  • HEK293 Cells
  • Humans
  • Neoplasms (drug therapy, genetics, pathology)
  • Oncogene Protein v-akt (genetics)
  • Phosphorylation (drug effects)
  • Plant Extracts (administration & dosage, chemistry)
  • Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
  • Proto-Oncogene Proteins c-fyn (genetics)
  • Signal Transduction (drug effects)
  • Swainsonine (administration & dosage)
  • bcl-2-Associated X Protein (biosynthesis)

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