Abstract | OBJECTIVES: The aim of this work was to assess left ventricular (LV) regional systolic function in rabbits with myocardial infarction after allogeneic mesenchymal stem cell (MSC) transplantation using quantitative tissue velocity imaging. METHODS: Thirty New Zealand White rabbits were assigned into 3 groups randomly: a sham-operated group (n = 10), a myocardial infarction (MI) group (n = 10), and a MSC transplantation group (n = 10). Mesenchymal stem cells (1 × 10(7) in total) were delivered into 5 spots around the left anterior descending artery (LAD) blood supply area via direct intramyocardial injections 1 hour after LAD ligation in the MSC group, whereas the MI group received the same amount of phosphate-buffered saline injections. Echocardiography was performed before LAD ligation and 1 day and 2 weeks after MSC transplantation, respectively. The peak systolic velocity (Vs) of each LV wall segment was measured. The myocardial slices were harvested for histologic staining after the last echocardiographic examination. RESULTS: The velocity curves for the LV myocardium before LAD ligation had a trend showing that the Vs value decreased gradually from basal to apical segments. The Vs values for the LV segments around the infarcted area in the MSC group decreased significantly compared with the sham group (P < .05) 1 day after MSC transplantation, whereas they increased significantly 2 weeks after MSC transplantation compared with 1 day after LAD ligation (P < .05). CONCLUSIONS: This study demonstrates that quantitative tissue velocity imaging may provide a promising approach to quantitatively assessing LV regional systolic function before and after MSC transplantation.
|
Authors | Shuangquan Jiang, Hairu Li, Min Ren, Jiawei Tian, Yanxin Su, Xiaoping Leng |
Journal | Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
(J Ultrasound Med)
Vol. 34
Issue 7
Pg. 1217-25
(Jul 2015)
ISSN: 1550-9613 [Electronic] England |
PMID | 26112624
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2015 by the American Institute of Ultrasound in Medicine. |
Topics |
- Animals
- Echocardiography, Doppler
- Heart Ventricles
(diagnostic imaging, physiopathology)
- Mesenchymal Stem Cell Transplantation
- Myocardial Infarction
(diagnostic imaging, physiopathology)
- Rabbits
- Ventricular Dysfunction, Left
(diagnostic imaging, physiopathology)
|