Oxymatrine ameliorates renal ischemia-reperfusion injury from oxidative stress through Nrf2/HO-1 pathway.

Abstract

PURPOSE:

To investigate if oxymatrine pretreatment could ameliorate renal I/R injury induced in rats and explore the possible role of oxymatrine in Nrf2/HO-1 pathway.

METHODS:

Unilaterally nephrectomized rats were insulted by I/R in their left kidney. Twenty four rats were randomly divided into three groups: sham group, I/R + saline-treated group, I/R + OMT-treated group. Oxymatrine or vehicle solution was administered intraperitoneally injected 60 min before renal ischemia, respectively. Renal function, histology, makers of oxidative stress, cell apoptosis and Nrf2/HO-1 expressions were assessed.

RESULTS:

Oxymatrine pretreatment exhibited an improved renal functional recovery, alleviated histological injury and oxidative stress, inhibiting tubular apoptosis, and accompanied by upregulated the expression of Nrf2/HO-1 proteins.

CONCLUSION:

Oxymatrine may attenuate renal ischemia/reperfusion injury, and this renoprotective effect may be through activating the Nrf2/HO-1 pathway.

• Authors: Guanjun Jiang, Xiuheng Liu, Min Wang, Hui Chen, Zhiyuan Chen, Tao Qiu
• Journal: Acta cirurgica brasileira (Acta Cir Bras) Vol. 30 Issue 6 Pg. 422-9 (Jun 2015) ISSN: 1678-2674 [Electronic] Brazil
• PMID: 26108031 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Alkaloids
• Antioxidants
• NF-E2-Related Factor 2
• Quinolizines
• oxymatrine
• Heme Oxygenase-1

Topics

• Alkaloids (pharmacology, therapeutic use)
• Animals
• Antioxidants (pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Blotting, Western
• Disease Models, Animal
• Heme Oxygenase-1 (analysis, metabolism)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Kidney (blood supply, pathology)
• Male
• NF-E2-Related Factor 2 (analysis, metabolism)
• Oxidative Stress (drug effects)
• Quinolizines (pharmacology, therapeutic use)
• Random Allocation
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Reproducibility of Results
• Time Factors
• Treatment Outcome