HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Partial Reversal of Obesity-Induced Insulin Resistance Owing to Anti-Inflammatory Immunomodulatory Potential of Flaxseed Oil.

Abstract
The present study was designed to assess the potential of supplementation of diet with Flaxseed (Linum usitatissimum, L.) oil (FXO), on obesity-related inflammation and reversal of obesity-induced insulin resistance. Swiss Albino mice, C57bl/6 mice and co-culture of 3T3-L1 adipocytes - RAW 264.7 macrophages to mimick obese adipose tissue environment were used for the study. Oral gavage of FXO at concentrations of 4, 8 or 16 mg/kg body weight (bwt) for 4 weeks or high-fat diet (HFD, 60% energy as fat) supplemented with dietary FXO (4, 8 or 16 mg/kg bwt) was given to the mice. FXO was characterised using gas chromatography - mass spectrometry. FXO supplemented HFD-fed mice (4 mg/kg bwt exhibited reduced adiposity index, serum glucose levels and triglycerides (8 and 16 mg/kg bwt) and improvement in insulin sensitisation (4, 8 and 16 mg/kg bwt) when compared with HFD mice. The co-culture showed a dose-dependent shift in cytokines towards anti-inflammatory (IL-4) state, with a decrease in pro-inflammatory TNF-α (p < 0.05). For immunomodulatory studies a dose-dependent increase (p < 0.05) was observed in antigen-specific levels of Th2 (IL-4) cytokine, serum anti-ova IgG1 and IgE levels. Suppression in anti-ova IgG2a, IgG2b, and IgG3 and antigen-specific Th1 cytokines like TNF-α and IFN-γ significantly (p < 0.05) was observed at 16 mg/kg bwt dosage. The results indicate that FXO exhibits an anti-inflammatory immunomodulatory potential and may partially relieve symptoms of obesity-associated insulin resistance.
AuthorsSamina Bashir, Shakir Ali, Farah Khan
JournalImmunological investigations (Immunol Invest) Vol. 44 Issue 5 Pg. 451-69 ( 2015) ISSN: 1532-4311 [Electronic] England
PMID26107745 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Inflammatory Agents
  • Cytokines
  • Immunoglobulin G
  • Immunologic Factors
  • Lipids
  • Immunoglobulin E
  • Linseed Oil
  • Ovalbumin
Topics
  • 3T3-L1 Cells
  • Adiposity (drug effects)
  • Animals
  • Anti-Inflammatory Agents (administration & dosage, pharmacology, therapeutic use)
  • Coculture Techniques
  • Cytokines (metabolism)
  • Diet, High-Fat
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Immunologic
  • Drug Evaluation, Preclinical
  • Glucose Tolerance Test
  • Hypersensitivity, Delayed (etiology, immunology, prevention & control)
  • Immunoglobulin E (immunology)
  • Immunoglobulin G (immunology)
  • Immunologic Factors (administration & dosage, pharmacology, therapeutic use)
  • Insulin Resistance
  • Linseed Oil (administration & dosage, pharmacology, therapeutic use)
  • Lipids (blood)
  • Liver (drug effects, pathology)
  • Lymphocyte Activation (drug effects)
  • Lymphoid Tissue (drug effects, pathology)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity (physiopathology)
  • Ovalbumin (immunology)
  • Phytotherapy
  • RAW 264.7 Cells
  • Th1 Cells (drug effects, metabolism)
  • Th2 Cells (drug effects, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: