Abstract |
Homocysteine thiolactone was demonstrated to inhibit the growth of 10-12-day-old chicken embryo cardiac tissue explants at 7 × 10⁻⁹ -1 × 10⁻³ M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10⁻³ M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10⁻³ M) and ouabain at concentrations regulating the signal-transducing (1 × 10⁻¹⁰ M) and pumping (1 × 10⁻⁸ M) functions of Na⁺,K⁺ - ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na⁺,K⁺ - ATPase pumping function.
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Authors | Ekaterina V Lopatina, A V Kipenko, V A Penniyaynen, N A Pasatetskaya, D Djuric, B V Krylov |
Journal | Acta physiologica Hungarica
(Acta Physiol Hung)
Vol. 102
Issue 2
Pg. 137-42
(Jun 2015)
ISSN: 0231-424X [Print] Hungary |
PMID | 26100303
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Homocysteine
- Ouabain
- homocysteine thiolactone
- Sodium-Potassium-Exchanging ATPase
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Topics |
- Animals
- Cell Proliferation
(drug effects)
- Chick Embryo
- Dose-Response Relationship, Drug
- Enzyme Inhibitors
(toxicity)
- Heart
(drug effects, embryology, growth & development)
- Homocysteine
(analogs & derivatives, toxicity)
- Myocytes, Cardiac
(drug effects, metabolism, pathology)
- Ouabain
(pharmacology)
- Signal Transduction
(drug effects)
- Sodium-Potassium-Exchanging ATPase
(antagonists & inhibitors, metabolism)
- Tissue Culture Techniques
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