Abstract |
Protection of telomere (Pot1) is a single-stranded telomere binding protein which is essential for chromosome ends protection. Fission yeast Rqh1 is a member of RecQ helicases family which has essential roles in the maintenance of genomic stability and regulation of homologous recombination. Double mutant between fission yeast pot1Δ and rqh1 helicase dead (rqh1-hd) maintains telomere by homologous recombination. In pot1Δ rqh1-hd double mutant, recombination intermediates accumulate near telomere which disturb chromosome segregation and make cells sensitive to microtubule inhibitors thiabendazole (TBZ). Deletion of chk1(+) or mutation of its kinase domain shortens the G2 of pot1Δ rqh1-hd double mutant and suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of that double mutant. In this study, we asked whether the long G2 is the reason for the TBZ sensitivity of pot1Δ rqh1-hd double mutant. We found that shortening the G2 of pot1Δ rqh1-hd double mutant by additional mutations of wee1 and mik1 or gain of function mutation of Cdc2 suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of pot1Δ rqh1-hd double mutant. Our results suggest that long G2 of pot1Δ rqh1-hd double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to TBZ.
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Authors | Ahmed G K Habib, Kenta Masuda, Masashi Yukawa, Eiko Tsuchiya, Masaru Ueno |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 464
Issue 1
Pg. 140-6
(Aug 14 2015)
ISSN: 1090-2104 [Electronic] United States |
PMID | 26093291
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Cell Cycle Proteins
- Nuclear Proteins
- Schizosaccharomyces pombe Proteins
- Shelterin Complex
- Telomere-Binding Proteins
- Tubulin Modulators
- pot1 protein, S pombe
- Protein Kinases
- mik1 protein, S pombe
- wee1 protein, S pombe
- Protein-Tyrosine Kinases
- Checkpoint Kinase 1
- Chk1 protein, S pombe
- CDC2 Protein Kinase
- cdc2 protein, S pombe
- DNA Helicases
- Rqh1 protein, S pombe
- Thiabendazole
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Topics |
- Binding Sites
- CDC2 Protein Kinase
(genetics, metabolism)
- Cell Cycle Proteins
(genetics, metabolism)
- Checkpoint Kinase 1
- Chromosome Segregation
(drug effects)
- Chromosomes, Fungal
(chemistry, drug effects, metabolism)
- DNA Helicases
(genetics, metabolism)
- G2 Phase
(drug effects, genetics)
- Gene Expression Regulation, Fungal
- Genome, Fungal
- Genomic Instability
- Homologous Recombination
(drug effects)
- Microbial Sensitivity Tests
- Microtubules
(chemistry, drug effects, metabolism)
- Mutation
- Nuclear Proteins
(genetics, metabolism)
- Protein Binding
- Protein Kinases
(deficiency, genetics)
- Protein-Tyrosine Kinases
(genetics, metabolism)
- Schizosaccharomyces
(drug effects, genetics, growth & development)
- Schizosaccharomyces pombe Proteins
(genetics, metabolism)
- Shelterin Complex
- Telomere
(chemistry, drug effects, metabolism)
- Telomere-Binding Proteins
(genetics, metabolism)
- Thiabendazole
(pharmacology)
- Tubulin Modulators
(pharmacology)
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