Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury.

Abstract

OBJECTIVE:

To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury.

METHODS:

36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-rich saline treatment group (I+HS). 30 min after injury, normal saline and hydrogen-rich saline were injected intraperitoneally (5 mL/kg) in I+NS group and I+HS group, respectively. All rats were euthanized and blood and organ specimens were collected for determination 24 h after inhalation injury.

RESULTS:

Tumor necrosis factor-alpha (TNF-α) levels, malondialdehyde (MDA) concentrations, nuclear factor kappa B (NF-κB) p65 expression, and apoptosis index (AI) in I+HS group were significantly decreased (P < 0.05), while superoxide dismutase (SOD) activities were increased compared with those in I+NS group; and a marked improvement in alveolar structure was also found after hydrogen-rich saline treatment.

CONCLUSIONS:

Hydrogen-rich saline treatment exerts protective effects in acute lung injury induced by inhalation injury, at least in part through the activation of anti-inflammatory and antioxidant pathways and inhibition of apoptosis.

• Authors: Xing Chen, Qi Liu, Dawei Wang, Shihai Feng, Yongjian Zhao, Yun Shi, Qun Liu
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2015 Pg. 106836 ( 2015) ISSN: 1942-0994 [Electronic] United States
• PMID: 26090070 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Protective Agents
• Transcription Factor RelA
• Tumor Necrosis Factor-alpha
• Sodium Chloride
• Malondialdehyde
• Hydrogen
• Superoxide Dismutase

Topics

• Animals
• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Hydrogen (pharmacology, therapeutic use)
• Lung (drug effects, metabolism, pathology, ultrastructure)
• Male
• Malondialdehyde (metabolism)
• Protective Agents (pharmacology, therapeutic use)
• Protein Transport (drug effects)
• Rats, Sprague-Dawley
• Smoke Inhalation Injury (blood, drug therapy, enzymology)
• Sodium Chloride (pharmacology, therapeutic use)
• Superoxide Dismutase (metabolism)
• Transcription Factor RelA (metabolism)
• Tumor Necrosis Factor-alpha (blood)