Abstract |
Effect of 5,5 diphenylhydantoin ( phenytoin; PHT) alone or in combination with epidermal growth factor ( EGF) on the intracellular accumulation of the radioisotope 45Ca2+ (4 min labelling period) was determined in gingival fibroblasts. EGF as well as PHT increased the intracellular accumulation of the radioisotope in normal gingival fibroblasts by approximately 2 and 1.6-fold, respectively. In contrast, in fibroblasts derived from the phenytoin-induced gingival overgrowth, neither EGF nor PHT stimulated intracellular accumulation of 45Ca2+. When normal gingival fibroblasts were treated in vitro with EGF in combination with PHT, the EGF-induced increase in intracellular accumulation of the radioisotope 45Ca2+ was abolished. The rate of efflux of the radioisotope 45Ca2+ in prelabelled normal gingival fibroblasts was decreased by PHT treatment in vitro to a level already present in fibroblasts derived from PHT-induced gingival overgrowth. This study indicates that PHT influences the cellular calcium metabolism in fibroblasts which may contribute to the pathogenesis of gingival overgrowth.
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Authors | G Brunius, T Modéer |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 18
Issue 8
Pg. 485-9
(Sep 1989)
ISSN: 0904-2512 [Print] Denmark |
PMID | 2607469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Calcium Radioisotopes
- Phenytoin
- Epidermal Growth Factor
- Calcium
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Topics |
- Adolescent
- Calcium
(pharmacokinetics)
- Calcium Radioisotopes
- Cells, Cultured
- Child
- Epidermal Growth Factor
(administration & dosage, pharmacology)
- Fibroblasts
(metabolism)
- Gingiva
(cytology, metabolism)
- Gingival Hyperplasia
(metabolism, pathology)
- Humans
- Phenytoin
(administration & dosage, pharmacology)
- Time Factors
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