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Effect of phenytoin on intracellular 45Ca2+ accumulation in gingival fibroblasts in vitro.

Abstract
Effect of 5,5 diphenylhydantoin (phenytoin; PHT) alone or in combination with epidermal growth factor (EGF) on the intracellular accumulation of the radioisotope 45Ca2+ (4 min labelling period) was determined in gingival fibroblasts. EGF as well as PHT increased the intracellular accumulation of the radioisotope in normal gingival fibroblasts by approximately 2 and 1.6-fold, respectively. In contrast, in fibroblasts derived from the phenytoin-induced gingival overgrowth, neither EGF nor PHT stimulated intracellular accumulation of 45Ca2+. When normal gingival fibroblasts were treated in vitro with EGF in combination with PHT, the EGF-induced increase in intracellular accumulation of the radioisotope 45Ca2+ was abolished. The rate of efflux of the radioisotope 45Ca2+ in prelabelled normal gingival fibroblasts was decreased by PHT treatment in vitro to a level already present in fibroblasts derived from PHT-induced gingival overgrowth. This study indicates that PHT influences the cellular calcium metabolism in fibroblasts which may contribute to the pathogenesis of gingival overgrowth.
AuthorsG Brunius, T Modéer
JournalJournal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 18 Issue 8 Pg. 485-9 (Sep 1989) ISSN: 0904-2512 [Print] Denmark
PMID2607469 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Calcium Radioisotopes
  • Phenytoin
  • Epidermal Growth Factor
  • Calcium
Topics
  • Adolescent
  • Calcium (pharmacokinetics)
  • Calcium Radioisotopes
  • Cells, Cultured
  • Child
  • Epidermal Growth Factor (administration & dosage, pharmacology)
  • Fibroblasts (metabolism)
  • Gingiva (cytology, metabolism)
  • Gingival Hyperplasia (metabolism, pathology)
  • Humans
  • Phenytoin (administration & dosage, pharmacology)
  • Time Factors

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