We devised a model system to study
persistent infection by the tick-borne flavivirus Langat virus (LGTV) in 293T cells.
Infection with a molecularly cloned LGTV strain produced an acute lytic crisis that left few surviving cells. The culture was repopulated by cells that were ~90% positive for LGTV E
protein, thus initiating a
persistent infection that was maintained for at least 35 weeks without additional lytic crises. Staining of cells for
viral proteins and ultrastructural analysis revealed only minor differences from the acute phase of
infection. Infectious LGTV decreased markedly over the study period, but the number of viral genomes remained relatively constant, suggesting the development of defective interfering particles (
DIPs). Viral genome changes were investigated by
RNA deep sequencing. At the initiation of
persistent infection, levels of
DIPs were below the limit of detection at a coverage depth of 11,288-fold, implying that
DIPs are not required for initiation of persistence. However, after 15 passages,
DIPs constituted approximately 34% of the total LGTV population (coverage of 1,293-fold). Furthermore, at this point, one specific DIP population predominated in which
nucleotides 1058 to 2881 had been deleted. This defective genome specified an intact
polyprotein that coded for a truncated fusion
protein containing 28 N-terminal residues of E and 134 C-terminal residues of NS1. Such a fusion
protein has not previously been described, and a possible function in
persistent infection is uncertain.
DIPs are not required for the initiation of persistent LGTV
infection but may play a role in the maintenance of viral persistence.
IMPORTANCE: Tick-borne flaviviruses are significant infectious agents that cause serious disease and death in humans worldwide.
Infections are characterized by severe neurological symptoms, such as
meningitis and
encephalitis. A high percentage of people who get infected and recuperate from the acute phase of
infection continue to suffer from chronic debilitating neurological sequelae, most likely as a result of nervous tissue damage, viral persistence, or both. However, little is known about mechanisms of viral persistence. Therefore, we undertook studies to investigate the persistence of Langat virus, a member of the tick-borne flavivirus group, in a mammalian cell line. Using next-generation sequencing, we determined that defective viral genomes do not play a role in the initiation of persistence, but their occurrence seems to be nonstochastic and could play a role in the maintenance of viral persistence via the expression of a novel envelope-NS1 fusion
protein.