Characteristic miR-24 Expression in Gastric Cancers among Atomic Bomb Survivors.

Abstract

OBJECTIVE:

To elucidate the mechanism of radiation-induced cancers, we analyzed the expression profiles of microRNAs extracted from formalin-fixed paraffin-embedded (FFPE) gastric cancer (GC) tissue samples from atomic bomb survivors.

METHODS:

The expression levels of miR-21, miR-24, miR-34a, miR-106a, miR-143, and miR-145 were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR).

RESULTS:

The expression of microRNAs was measured by qRT-PCR in a Hiroshima University Hospital cohort comprising 32 patients in the high-dose-exposed group and 18 patients in the low-dose-exposed group who developed GC after the bombing. The GC cases showing high expression of miR-24, miR-143, and miR-145 were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. We next performed qRT-PCR of miR-24, miR-143, and miR-145 in a cohort from the Hiroshima Red Cross Hospital and Atomic-Bomb Survivors Hospital comprising 122 patients in the high-dose-exposed group and 48 patients in the low-dose-exposed group who developed GC after the bombing. High expressions of miR-24 and miR-143 were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. Multivariate analysis demonstrated that only high expression of miR-24 was an independent predictor for the exposure status.

CONCLUSION:

These results suggest that the measurement of miR-24 expression from FFPE samples is useful to identify radiation-associated GC.

• Authors: Yutaka Naito, Naohide Oue, Trang T B Pham, Manabu Yamamoto, Megumu Fujihara, Teruyoshi Ishida, Shoichiro Mukai, Kazuhiro Sentani, Naoya Sakamoto, Eisuke Hida, Hiroki Sasaki, Wataru Yasui
• Journal: Pathobiology : journal of immunopathology, molecular and cellular biology (Pathobiology) Vol. 82 Issue 2 Pg. 68-75 ( 2015) ISSN: 1423-0291 [Electronic] Switzerland
• PMID: 26045155 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• MIRN24 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Biomarkers
• Female
• Humans
• Male
• MicroRNAs (genetics)
• Middle Aged
• Neoplasms, Radiation-Induced (genetics)
• Nuclear Weapons
• Real-Time Polymerase Chain Reaction
• Retrospective Studies
• Stomach Neoplasms (genetics)
• Survivors