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Cuprizone Model as a Tool for Preclinical Studies of the Efficacy of Multiple Sclerosis Diagnosis and Therapy.

Abstract
To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy.
AuthorsT O Abakumova, A A Kuz'kina, M E Zharova, M V Zharova, D A Pozdeeva, I L Gubskii, I I Shepeleva, O M Antonova, N V Nukolova, Z I Kekelidze, V P Chekhonin
JournalBulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 159 Issue 1 Pg. 111-5 (May 2015) ISSN: 1573-8221 [Electronic] United States
PMID26028235 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Glial Fibrillary Acidic Protein
  • Nerve Tissue Proteins
  • glial fibrillary astrocytic protein, mouse
  • Cuprizone
Topics
  • Animals
  • Brain (diagnostic imaging)
  • Cuprizone (pharmacology)
  • Demyelinating Diseases (chemically induced)
  • Disease Models, Animal
  • Female
  • Glial Fibrillary Acidic Protein
  • Magnetic Resonance Imaging
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Multiple Sclerosis (chemically induced, therapy)
  • Myelin Sheath (metabolism)
  • Nerve Tissue Proteins (metabolism)
  • Radiography

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