The novel HDAC inhibitor AR-42-induced anti-colon cancer cell activity is associated with ceramide production.
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| Abstract |
In the current study, we investigated the potential activity of AR-42, a novel histone deacetylase (HDAC) inhibitor, against colon cancer cells. Our in vitro results showed that AR-42 induced ceramide production, exerted potent anti-proliferative and pro-apoptotic activities in established (SW-620 and HCT-116 lines) and primary human colon cancer cells. Exogenously-added sphingosine 1-phosphate (S1P) suppressed AR-42-induced activity, yet a cell-permeable ceramide (C4) facilitated AR-42-induced cytotoxicity against colon cancer cells. In addition, AR-42-induced ceramide production and anti-colon cancer cell activity were inhibited by the ceramide synthase inhibitor fumonisin B1, but were exacerbated by PDMP, which is a ceramide glucosylation inhibitor. In vivo, oral administration of a single dose of AR-42 dramatically inhibited SW-620 xenograft growth in severe combined immunodeficient (SCID) mice, without inducing overt toxicities. Together, these results show that AR-42 dramatically inhibits colon cancer cell proliferation in vitro and in vivo, and ceramide production might be the key mechanism responsible for its actions.
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| Authors | Weihong Xu, Bin Xu, Yiting Yao, Xiaoling Yu, Jie Shen |
| Journal | Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 463 Issue 4 Pg. 545-50 (Aug 07 2015) ISSN: 1090-2104 [Electronic] United States |
| PMID | 26026677 (Publication Type: Journal Article) |
| Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
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