Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.

Abstract

AIM:

Peripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor type 4 antagonist AMD3100, as well as via a diluted whole blood incubation (DWBI).

MATERIALS & METHODS:

Porcine pBD-ECs were isolated using AMD3100 and DWBI and tested for EC markers, acetylated LDL uptake, growth kinetics, metabolic activity, flow-mediated nitric oxide production and seeded onto titanium tubes implanted into vessels of pigs.

RESULTS:

DWBI increased the yield of porcine pBD-ECs 6.6-fold, and AMD3100 increased the yield 4.5-fold. AMD3100-mobilized ECs were phenotypically indistinguishable from nonmobilized ECs. In porcine implants, the cells expressed endothelial nitric oxide synthase, reduced thrombin-antithrombin complex systemically and prevented thrombosis.

CONCLUSION:

Administration of AMD3100 and the DWBI method both increase pBD-EC yield.

• Authors: Ryan M Jamiolkowski, Sa Do Kang, AnnMarie K Rodriguez, Justin M Haseltine, Lauren J Galinat, Alexandra E Jantzen, Tim A Carlon, Marcus D Darrabie, Antonio J Arciniegas, Jose G Mantilla, N Rebecca Haley, Maria Noviani, Jason D Allen, Thomas V Stabler, James W Frederiksen, Oscar Alzate, Lukas G Keil, Siyao Liu, Fu-Hsiung Lin, George A Truskey, Hardean E Achneck
• Journal: Regenerative medicine (Regen Med) Vol. 10 Issue 4 Pg. 447-60 (May 2015) ISSN: 1746-076X [Electronic] England
• PMID: 26022764 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Benzylamines
• Cyclams
• Heterocyclic Compounds
• plerixafor

Topics

• Animals
• Benzylamines
• Cell Separation
• Cell Transplantation (methods)
• Cyclams
• Endothelial Cells (cytology, drug effects)
• Flow Cytometry
• Heterocyclic Compounds (administration & dosage, pharmacology)
• Models, Animal
• Rheology (drug effects)
• Stress, Mechanical
• Sus scrofa
• Tissue Engineering (methods)
• Transplantation, Autologous
• Vena Cava, Inferior (drug effects, physiology)