The novel secretory protein CGREF1 inhibits the activation of AP-1 transcriptional activity and cell proliferation.

Abstract

The transcription factor AP-1 plays an important role in inflammation and cell survival. Using a dual-luciferase reporter assay system and a library of 940 candidate human secretory protein cDNA clones, we identified that CGREF1 can inhibit the transcriptional activity of AP-1. We demonstrated that CGREF1 is secreted via the classical secretory pathway through the ER-to-Golgi apparatus. Functional investigations revealed that overexpression of CGREF1 can significantly inhibit the phosphorylation of ERK and p38 MAPK, and suppress the proliferation of HEK293T and HCT116 cells. Conversely, specific siRNAs against CGREF1 can increase the transcriptional activity of AP-1. These results clearly indicated that CGREF1 is a novel secretory protein, and plays an important role in regulation of AP-1 transcriptional activity and cell proliferation.

• Authors: Weiwei Deng, Lan Wang, Ying Xiong, Jing Li, Ying Wang, Taiping Shi, Dalong Ma
• Journal: The international journal of biochemistry & cell biology (Int J Biochem Cell Biol) Vol. 65 Pg. 32-9 (Aug 2015) ISSN: 1878-5875 [Electronic] Netherlands
• PMID: 26022276 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• CGREF1 protein, human
• Calcium-Binding Proteins
• Transcription Factor AP-1
• Mitogen-Activated Protein Kinase Kinases

Topics

• Calcium-Binding Proteins (genetics, metabolism)
• Cell Proliferation (physiology)
• HEK293 Cells
• HeLa Cells
• Humans
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Phosphorylation
• Signal Transduction
• Transcription Factor AP-1 (antagonists & inhibitors, genetics, metabolism)
• Transfection