Role of the brain stem in tibial inhibition of the micturition reflex in cats.

Abstract

This study examined the role of the brain stem in inhibition of bladder reflexes induced by tibial nerve stimulation (TNS) in α-chloralose-anesthetized decerebrate cats. Repeated cystometrograms (CMGs) were performed by infusing saline or 0.25% acetic acid (AA) to elicit normal or overactive bladder reflexes, respectively. TNS (5 or 30 Hz) at three times the threshold (3T) intensity for inducing toe movement was applied for 30 min between CMGs to induce post-TNS inhibition or applied during the CMGs to induce acute TNS inhibition. Inhibition was evident as an increase in bladder capacity without a change in amplitude of bladder contractions. TNS applied for 30 min between saline CMGs elicited prolonged (>2 h) poststimulation inhibition that significantly (P < 0.05) increased bladder capacity to 30-60% above control; however, TNS did not produce this effect during AA irritation. TNS applied during CMGs at 5 Hz but not 30 Hz significantly (P < 0.01) increased bladder capacity to 127.3 ± 6.1% of saline control or 187.6 ± 5.0% of AA control. During AA irritation, naloxone (an opioid receptor antagonist) administered intravenously (1 mg/kg) or directly to the surface of the rostral brain stem (300-900 μg) eliminated acute TNS inhibition and significantly (P < 0.05) reduced bladder capacity to 62.8 ± 22.6% (intravenously) or 47.6 ± 25.5% (brain stem application). Results of this and previous studies indicate 1) forebrain circuitry rostral to the pons is not essential for TNS inhibition; and 2) opioid receptors in the brain stem have a critical role in TNS inhibition of overactive bladder reflexes but are not involved in inhibition of normal bladder reflexes.

• Authors: Matthew C Ferroni, Rick C Slater, Bing Shen, Zhiying Xiao, Jicheng Wang, Andy Lee, James R Roppolo, William C de Groat, Changfeng Tai
• Journal: American journal of physiology. Renal physiology (Am J Physiol Renal Physiol) Vol. 309 Issue 3 Pg. F242-50 (Aug 01 2015) ISSN: 1522-1466 [Electronic] United States
• PMID: 26017973 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2015 the American Physiological Society.

Chemical References

• Narcotic Antagonists
• Naloxone
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Brain Stem (physiology)
• Cats
• Decerebrate State (physiopathology)
• Electric Stimulation
• Female
• Male
• Naloxone (pharmacology)
• Narcotic Antagonists (pharmacology)
• Pudendal Nerve (drug effects)
• Reflex (physiology)
• Tibial Nerve (physiology)
• Urinary Bladder (innervation, physiopathology)
• Urinary Bladder, Overactive (chemically induced, physiopathology)
• Urination (physiology)