Abstract | BACKGROUND: Tendon hydrogel is a promising new injectable substance that has been shown to improve repair strength after tendon injury. This study assesses the capacity of platelet-rich plasma to stimulate proliferation and migration of rat adipose-derived stem cells in tendon hydrogel in vitro. METHODS: To assess proliferation, adipose-derived stem cells were exposed to plasma, plasma supplemented with growth factors, or platelet-rich plasma in culture medium and tendon hydrogel. To assess migration, adipose-derived stem cells were plated onto tendon hydrogel -coated wells and covered with medium containing plasma, plasma supplemented with growth factors, platelet-rich plasma, or bovine serum albumin. Migration from cell-seeded to cell-free zones was assessed at 12-hour intervals. RESULTS: Platelet-rich plasma augmented proliferation to a greater extent compared with plasma and plasma supplemented with growth factors (10%: optical density, 1.18 versus 0.75 versus 0.98, respectively). Platelet-rich plasma was superior to plasma in tendon hydrogel (10%: optical density, 1.19 versus 0.85) but did not augment proliferation to the extent that plasma supplemented with growth factors did (10%: optical density, 1.19 versus 1.56). Platelet-rich plasma enhanced the migration of adipose-derived stem cells compared with serum-free medium ( bovine serum albumin) (36 hours: platelet-rich plasma, 1.88; plasma, 1.51; plasma plus growth factor, 1.80; bovine serum albumin, 1.43). CONCLUSIONS: Tendon healing is mediated by migration of cells to the injured area and cellular proliferation at that site. Tendon hydrogel supplemented with platelet-rich plasma stimulates these processes. Future studies will evaluate this combination's ability to stimulate healing in chronic tendon injuries in vivo.
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Authors | Christopher S Crowe, Grace Chiou, Rory McGoldrick, Kenneth Hui, Hung Pham, James Chang |
Journal | Plastic and reconstructive surgery
(Plast Reconstr Surg)
Vol. 135
Issue 6
Pg. 981e-989e
(Jun 2015)
ISSN: 1529-4242 [Electronic] United States |
PMID | 26017614
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media
- Hydrogel, Polyethylene Glycol Dimethacrylate
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Topics |
- Adipocytes
(transplantation)
- Animals
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Culture Media
- Hydrogel, Polyethylene Glycol Dimethacrylate
(pharmacology)
- In Vitro Techniques
- Models, Animal
- Platelet-Rich Plasma
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Regeneration
(physiology)
- Sensitivity and Specificity
- Stem Cells
(cytology, physiology)
- Tendons
(physiology, surgery)
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