Abstract | BACKGROUND: METHODS: The authors used their Achilles tenotomy and burn model to evaluate the osteogenic potential of mesenchymal stem cells of male and female injured and noninjured mice. Groups consisted of injured male (n = 3), injured female (n = 3), noninjured male (n = 3), and noninjured female (n = 3) mice. The osteogenic potential of cells harvested from each group was assessed through RNA and protein levels and quantified using micro-computed tomographic scan. Histomorphometry was used to verify micro-computed tomographic findings, and immunohistochemistry was used to assess osteogenic signaling at the site of heterotopic ossification. RESULTS: Mesenchymal stem cells of male mice demonstrated greater osteogenic gene and protein expression than those of female mice (p < 0.05). Male mice in the burn group formed 35 percent more bone than female mice in the burn group. This bone formation correlated with increased pSmad and insulin-like growth factor 1 signaling at the heterotopic ossification site in male mice. CONCLUSIONS:
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Authors | Kavitha Ranganathan, Jonathan Peterson, Shailesh Agarwal, Eboda Oluwatobi, Shawn Loder, Jonathan A Forsberg, Thomas A Davis, Steven R Buchman, Stewart C Wang, Benjamin Levi |
Journal | Plastic and reconstructive surgery
(Plast Reconstr Surg)
Vol. 135
Issue 6
Pg. 1631-1641
(Jun 2015)
ISSN: 1529-4242 [Electronic] United States |
PMID | 26017598
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bone Morphogenetic Proteins
- Insulin-Like Growth Factor I
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Topics |
- Achilles Tendon
(injuries, surgery)
- Analysis of Variance
- Animals
- Biopsy, Needle
- Blotting, Western
- Bone Morphogenetic Proteins
(metabolism)
- Burns
(complications, pathology)
- Disease Models, Animal
- Female
- Immunohistochemistry
- Insulin-Like Growth Factor I
(metabolism)
- Male
- Mesenchymal Stem Cells
(pathology)
- Mice
- Mice, Inbred Strains
- Ossification, Heterotopic
(etiology, pathology)
- Osteogenesis
(physiology)
- Polymerase Chain Reaction
- Random Allocation
- Risk Assessment
- Sex Factors
- Tenotomy
(methods)
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