The role of 8-OH-DPAT on the rat neuronal apoptosis after diffuse brain injury coupled with secondary brain injury.

Abstract

The potential role of 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) on rat neuronal apoptosis after diffuse brain injury (DBI) coupled with secondary brain injury (SBI) was investigated. One hundred and twelve adult male Wister rats weighing 305-355 g were randomly divided into four groups and received an intraperitoneal injection of 8-OH-DPAT (0.5 mg/kg) or an equal volume of normal saline. Neurological severity score (NSS) was recorded and the injured extent was observed after hematoxylin-eosin (HE) staining. The neuronal cell apoptosis index and the expression of Bax and Bcl-2 were detected by TUNEL method and immunohistochemistry respectively. We found a higher NSS value for rats in the DBI + SBI groups compared with those in normal control and sham-operated control groups (P < 0.01). HE staining showed that 8-OH-DPAT treatment could alleviate the occurrence of injury in rats CA3 hippocampus and PFC. The neuronal apoptosis index decreased in the 8-OH-DPAT treatment group compared with the NS group (P< 0.05) and gradually increased at 6 h, reached the peak level at 72 h and still had a high performance at 168 h in not only CA3 hippocampus but also PFC. Expression of Bax and Bcl-2 increased after DBI + SBI, however, with 8-OH-DPAT treatment Bcl-2 expression increased while Bax expression decreased. 8-OH-DPAT had an inhibitory effect on the rat neuronal apoptosis in CA3 hippocampus and PFC after DBI coupled with SBI.

• Authors: Bin Li, Zhenquan Song, Guobiao Liang, Wei Lei, Zhijun Wang, Enzhi Liu, Xiaoming Li, Hongbo Liu
• Journal: Die Pharmazie (Pharmazie) Vol. 70 Issue 4 Pg. 251-5 (Apr 2015) ISSN: 0031-7144 [Print] Germany
• PMID: 26012255 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bax protein, rat
• Proto-Oncogene Proteins c-bcl-2
• Serotonin Receptor Agonists
• bcl-2-Associated X Protein
• 8-Hydroxy-2-(di-n-propylamino)tetralin

Topics

• 8-Hydroxy-2-(di-n-propylamino)tetralin (pharmacology)
• Animals
• Apoptosis (drug effects)
• Brain Injuries (drug therapy, pathology)
• CA3 Region, Hippocampal (drug effects, pathology)
• Male
• Neurons (drug effects)
• Prefrontal Cortex (drug effects, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Rats
• Rats, Wistar
• Serotonin Receptor Agonists (pharmacology)
• Treatment Outcome
• bcl-2-Associated X Protein (metabolism)