HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Color-Coded Imaging of Breast Cancer Metastatic Niche Formation in Nude Mice.

Abstract
We report here a color-coded imaging model in which metastatic niches in the lung and liver of breast cancer can be identified. The transgenic green fluorescent protein (GFP)-expressing nude mouse was used as the host. The GFP nude mouse expresses GFP in all organs. However, GFP expression is dim in the liver parenchymal cells. Mouse mammary tumor cells (MMT 060562) (MMT), expressing red fluorescent protein (RFP), were injected in the tail vein of GFP nude mice to produce experimental lung metastasis and in the spleen of GFP nude mice to establish a liver metastasis model. Niche formation in the lung and liver metastasis was observed using very high resolution imaging systems. In the lung, GFP host-mouse cells accumulated around as few as a single MMT-RFP cell. In addition, GFP host cells were observed to form circle-shaped niches in the lung even without RFP cancer cells, which was possibly a niche in which future metastasis could be formed. In the liver, as with the lung, GFP host cells could form circle-shaped niches. Liver and lung metastases were removed surgically and cultured in vitro. MMT-RFP cells and GFP host cells resembling cancer-associated fibroblasts (CAFs) were observed interacting, suggesting that CAFs could serve as a metastatic niche.
AuthorsAtsushi Suetsugu, Masashi Momiyama, Yukihiko Hiroshima, Masahito Shimizu, Shigetoyo Saji, Hisataka Moriwaki, Michael Bouvet, Robert M Hoffman
JournalJournal of cellular biochemistry (J Cell Biochem) Vol. 116 Issue 12 Pg. 2730-4 (Dec 2015) ISSN: 1097-4644 [Electronic] United States
PMID25981355 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Copyright© 2015 Wiley Periodicals, Inc.
Chemical References
  • Luminescent Proteins
  • red fluorescent protein
  • Green Fluorescent Proteins
Topics
  • Animals
  • Breast Neoplasms (genetics, metabolism, pathology)
  • Female
  • Green Fluorescent Proteins (metabolism)
  • Humans
  • Liver Neoplasms (genetics, pathology, secondary)
  • Luminescent Proteins (metabolism)
  • Mice
  • Mice, Nude
  • Molecular Imaging (methods)
  • Neoplasm Metastasis
  • Tumor Microenvironment (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: