Abstract | CONTEXT: OBJECTIVE: The purpose of this study was to further characterize the effects of H2-rich water (0.9 L/day) on the content, composition, and biological activities of plasma lipoproteins on patients with hypercholesterolemia and their underlying mechanisms in a double-blinded, randomized, and placebo-controlled trial. DESIGN: This was a case-control study. SETTING: The setting was the Zhoudian community, Tai'an, China. PATIENTS: A total of 68 patients with untreated isolated hypercholesterolemia were randomly allocated to either drinking H2-rich water (n = 34) or placebo water (n = 34) for 10 weeks. RESULTS: HDL isolated from the H2 group showed an increased ability to promote the ATP-binding cassette transporter A1-mediated cholesterol efflux ex vivo. Plasma pre-β-HDL levels were up-regulated although there were no changes in plasma HDL-cholesterol levels. Moreover, other HDL functions, assessed in protection against LDL oxidation, inhibition of oxidized-LDL-induced inflammation, and protection of endothelial cells from oxidized-LDL-induced apoptosis, were all significantly improved by H2 treatment. In addition, H2 treatment increased the effective rate in down-regulating plasma levels of total cholesterol (47.06% vs 17.65%) and LDL cholesterol (47.06% vs 23.53%). Western blot analysis revealed a marked decrease in apolipoprotein B100 and an increase in apolipoprotein M in plasma of the H2 group. Finally H2 treatment resulted in a significant reduction in the levels of several inflammatory and oxidative stress indicators in whole plasma and HDL particles. CONCLUSIONS:
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Authors | Guohua Song, Quanqiang Lin, Hui Zhao, Meiyuan Liu, Fenglong Ye, Yujuan Sun, Yang Yu, Shoudong Guo, Peng Jiao, Yun Wu, Guoyong Ding, Qiang Xiao, Shucun Qin |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 100
Issue 7
Pg. 2724-33
(Jul 2015)
ISSN: 1945-7197 [Electronic] United States |
PMID | 25978109
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- ABCA1 protein, human
- ATP Binding Cassette Transporter 1
- Lipoproteins, HDL
- Placebos
- Hydrogen
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Topics |
- ATP Binding Cassette Transporter 1
(metabolism)
- Adult
- Animals
- Biological Transport
(drug effects)
- Case-Control Studies
- Cells, Cultured
- China
- Double-Blind Method
- Humans
- Hydrogen
(pharmacology)
- Hypercholesterolemia
(blood, metabolism)
- Lipoproteins, HDL
(metabolism, physiology)
- Macrophages
(drug effects, metabolism)
- Mice
- Middle Aged
- Oxidative Stress
(drug effects)
- Placebos
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