Monascus pilosus-fermented black soybean inhibits lipid accumulation in adipocytes and in high-fat diet-induced obese mice.

Abstract

OBJECTIVE:

To explore the anti-obesity effects and the mechanism of action of Monascus pilosus(M. pilosus)-fermented black soybean (MFBS) extracts (MFBSE) and MFBS powders (MFBSP) in adipocytes and high-fat diet (HFD)-induced obese mice, respectively.

METHODS:

Black soybean was fermented with M. pilosus, and the main constituents in MFBS were analyzed by HPLC analysis. In vitro, MFBSE were examined for anti-adipogenic effects using Oil-Red O staining. In vivo, mice were fed a normal-fat diet (NFD) control, HFD control or HFD containing 1 g/kg MFBSP for 12 weeks, and then body weight gain and tissues weight measured. Real-time PCR and western blot assay were used to determine the mechanism of anti-adipogenic effects.

RESULTS:

MFBSE inhibited lipid accumulation in 3T3-L1 adipocytes without exerting cell cytotoxicity. MFBSP treatment in HFD-fed mice significantly decreased the body weight gain compared with the HFD control mice. MFBSE and MFBSP treatment resulted in significantly lower mRNA levels of adipogenesis-related genes, such as peroxisome proliferator-activated receptor γ(PPAR γ), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS), in adipocytes and in white adipose tissue (WAT) of HFD-induced obese mice.

CONCLUSIONS:

These results suggest that the anti-obesity effects of MFBS are elicited by regulating the expression of adipogenesis-related genes in adipocytes and WAT of HFD-induced obese mice.

• Authors: Young-Sil Lee, Bong-Keun Choi, Hae Jin Lee, Dong-Ryung Lee, Jinhua Cheng, Won-Keun Lee, Seung Hwan Yang, Joo-Won Suh
• Journal: Asian Pacific journal of tropical medicine (Asian Pac J Trop Med) Vol. 8 Issue 4 Pg. 276-82 (Apr 2015) ISSN: 2352-4146 [Electronic] India
• PMID: 25975498 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.