Abstract | BACKGROUND:
Photodynamic therapy ( PDT) combines light, molecular oxygen and a photosensitizer to induce oxidative stress in target cells. Certain hydrophobic photosensitizers, such as aluminium- phthalocyanine chloride (AlPc), have significant potential for antitumor PDT applications. However, hydrophobic molecules often require drug-delivery systems, such as nanostructures, to improve their pharmacokinetic properties and to prevent aggregation, which has a quenching effect on the photoemission properties in aqueous media. As a result, this work aims to develop and test the efficacy of an AlPc in the form of a nanoemulsion to enable its use in anticancer PDT. RESULTS: The nanoemulsion was developed using castor oil and Cremophor ELP®, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 25 nm was obtained. While free AlPc failed to show significant activity against human breast adenocarcinoma MCF-7 cells in an in vitro PDT assay, the AlPc in the nanoemulsion showed intense photodynamic activity. Photoactivated AlPc exhibited a 50 % cytotoxicity concentration (CC50) of 6.0 nM when applied to MCF-7 cell monolayers and exerted a powerful cytotoxic effect on MCF-7 cell spheroids. CONCLUSION: Through the use of spontaneous emulsification, a stable AlPc nanoemulsion was developed that exhibits strong in vitro photodynamic activity on cancer cells.
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Authors | Luis Alexandre Muehlmann, Mosar Corrêa Rodrigues, João Paulo Figueiró Longo, Mônica Pereira Garcia, Karen Rapp Py-Daniel, Aline Bessa Veloso, Paulo Eduardo Narciso de Souza, Sebastião William da Silva, Ricardo Bentes Azevedo |
Journal | Journal of nanobiotechnology
(J Nanobiotechnology)
Vol. 13
Pg. 36
(May 13 2015)
ISSN: 1477-3155 [Electronic] England |
PMID | 25966866
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Colloids
- Emulsions
- Indoles
- Isoindoles
- Surface-Active Agents
- Castor Oil
- Aluminum
- phthalocyanine
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Topics |
- Aluminum
(chemistry, pharmacology)
- Antineoplastic Agents
(chemistry, pharmacology)
- Castor Oil
(chemistry)
- Colloids
(chemistry)
- Dose-Response Relationship, Drug
- Drug Delivery Systems
- Emulsions
(chemistry, pharmacology)
- Humans
- Indoles
(chemistry, pharmacology)
- Isoindoles
- MCF-7 Cells
(drug effects, pathology)
- Nanostructures
(chemistry)
- Photochemotherapy
(methods)
- Spectrum Analysis, Raman
- Surface-Active Agents
(chemistry)
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