Inhibition of miR-146b expression increases radioiodine-sensitivity in poorly differential thyroid carcinoma via positively regulating NIS expression.

Abstract

Dedifferentiated thyroid carcinoma (DTC) with the loss of radioiodine uptake (RAIU) is often observed in clinical practice under radioiodine therapy, indicating the challenge for poor prognosis. MicroRNA (miRNA) has emerged as a promising therapeutic target in many diseases; yet, the role of miRNAs in RAIU has not been generally investigated. Based on recent studies about miRNA expression in papillary or follicular thyroid carcinomas, the expression profiles of several thyroid relative miRNAs were investigated in one DTC cell line, derived from normal DTC cells by radioiodine treatment. The top candidate miR-146b, with the most significant overexpression profiles in dedifferentiated cells, was picked up. Further research found that miR-146b could be negatively regulated by histone deacetylase 3 (HDAC3) in normal cells, indicating the correlation between miR-146b and Na(+)/I(-) symporter (NIS)-mediated RAIU. Fortunately, it was confirmed that miR-146b could regulate NIS expression/activity; what is more important, miR-146b interference would contribute to the recovery of radioiodine-sensitivity in dedifferentiated cells via positively regulating NIS. In the present study, it was concluded that NIS-mediated RAIU could be modulated by miR-146b; accordingly, miR-146b might serve as one of targets to enhance efficacy of radioactive therapy against poorly differential thyroid carcinoma (PDTC).

• Authors: Luchuan Li, Bin Lv, Bo Chen, Ming Guan, Yongfeng Sun, Haipeng Li, Binbin Zhang, Changyuan Ding, Shan He, Qingdong Zeng
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 462 Issue 4 Pg. 314-21 (Jul 10 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25960292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• DNA Primers
• Iodine Radioisotopes
• MIRN146 microRNA, human
• MicroRNAs
• Symporters
• sodium-iodide symporter
• Histone Deacetylases

Topics

• Base Sequence
• Cell Differentiation
• DNA Primers
• Histone Deacetylases (metabolism)
• Humans
• Iodine Radioisotopes (pharmacology)
• MicroRNAs (genetics)
• Real-Time Polymerase Chain Reaction
• Symporters (genetics)
• Thyroid Neoplasms (genetics, pathology)