Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis.

Abstract

Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5'-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5'-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron.

• Authors: Patrícia de Brum Vieira, Nícolas Luiz Feijó Silva, Luiza Wilges Kist, Giovanna Medeiros Tavares de Oliveira, Maurício Reis Bogo, Geraldo Atillio de Carli, Alexandre José Macedo, Tiana Tasca
• Journal: Memorias do Instituto Oswaldo Cruz (Mem Inst Oswaldo Cruz) Vol. 110 Issue 2 Pg. 201-8 (Apr 2015) ISSN: 1678-8060 [Electronic] Brazil
• PMID: 25946243 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemoglobins
• Nucleotides
• RNA, Protozoan
• Adenosine Monophosphate
• Adenosine Diphosphate
• Hemin
• Adenosine Triphosphate
• Iron
• 5'-Nucleotidase
• Adenosine Triphosphatases

Topics

• 5'-Nucleotidase (metabolism)
• Adenosine Diphosphate (metabolism)
• Adenosine Monophosphate (metabolism)
• Adenosine Triphosphatases (metabolism)
• Adenosine Triphosphate (metabolism)
• Cell Culture Techniques
• Female
• Gene Expression (drug effects)
• Hemin (chemistry)
• Hemoglobins (chemistry)
• Humans
• Hydrolysis
• Iron (administration & dosage, physiology)
• Male
• Nucleotides (metabolism)
• RNA, Protozoan
• Reverse Transcriptase Polymerase Chain Reaction
• Trichomonas Infections (enzymology)
• Trichomonas vaginalis (drug effects, enzymology, growth & development)