In Vivo Molecular MRI Imaging of Prostate Cancer by Targeting PSMA with Polypeptide-Labeled Superparamagnetic Iron Oxide Nanoparticles.

Abstract	The prostate specific membrane antigen (PSMA) is broadly overexpressed on prostate cancer (PCa) cell surfaces. In this study, we report the synthesis, characterization, in vitro binding assay, and in vivo magnetic resonance imaging (MRI) evaluation of PSMA targeting superparamagnetic iron oxide nanoparticles (SPIONs). PSMA-targeting polypeptide CQKHHNYLC was conjugated to SPIONs to form PSMA-targeting molecular MRI contrast agents. In vitro studies demonstrated specific uptake of polypeptide-SPIONs by PSMA expressing cells. In vivo MRI studies found that MRI signals in PSMA-expressing tumors could be specifically enhanced with polypeptide-SPION, and further Prussian blue staining showed heterogeneous deposition of SPIONs in the tumor tissues. Taken altogether, we have developed PSMA-targeting polypeptide-SPIONs that could specifically enhance MRI signal in tumor-bearing mice, which might provide a new strategy for the molecular imaging of PCa.
Authors	Yunkai Zhu, Ying Sun, Yaqing Chen, Weiyong Liu, Jun Jiang, Wenbin Guan, Zhongyang Zhang, Yourong Duan
Journal	International journal of molecular sciences (Int J Mol Sci) Vol. 16 Issue 5 Pg. 9573-87 (Apr 28 2015) ISSN: 1422-0067 [Electronic] Switzerland
PMID	25927579 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, Surface Contrast Media Ferric Compounds Ferrocyanides Peptides ferric oxide Polylactic Acid-Polyglycolic Acid Copolymer Polyglycolic Acid Lactic Acid FOLH1 protein, human Glutamate Carboxypeptidase II ferric ferrocyanide
Topics	Animals Antigens, Surface (chemistry) Cell Line, Tumor Contrast Media (chemistry) Ferric Compounds (chemistry) Ferrocyanides (chemistry) Glutamate Carboxypeptidase II (chemistry) Humans Lactic Acid (chemistry) Magnetic Resonance Imaging Male Metal Nanoparticles (chemistry) Mice Mice, Inbred BALB C Mice, Nude Nanotechnology Neoplasm Transplantation Peptides (chemistry) Polyglycolic Acid (chemistry) Polylactic Acid-Polyglycolic Acid Copolymer Prostatic Neoplasms (diagnosis, pathology) Protein Binding Signal Transduction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!